Search results for "Bone cell"

showing 10 items of 22 documents

Fabrication of amorphous strontium polyphosphate microparticles that induce mineralization of bone cells in vitro and in vivo.

2017

Abstract Here we describe the fabrication process of amorphous strontium-polyphosphate microparticles (“Sr-a-polyP-MP”). The effects of these particles on growth and gene expression were investigated with SaOS-2 cells as well as with human mesenchymal stem cells (MSC) and compared with those particles prepared of amorphous calcium-polyphosphate (“Ca-a-polyP-MP”) and of strontium salt. The results revealed a markedly higher stimulation of growth of MSC by “Sr-a-polyP-MP” compared to “Ca-a-polyP-MP” and a significant increase in mineralization of SaOS-2 cells, as well as an enhanced upregulation of the expression of the genes encoding for alkaline phosphatase and the bone morphogenetic protei…

0301 basic medicineMaterials scienceBiomedical Engineering02 engineering and technologyBone healingBiochemistryBone morphogenetic protein 2OsteocytesBiomaterials03 medical and health scienceschemistry.chemical_compoundCalcification PhysiologicIn vivoPolyphosphatesCell Line TumorBone cellAnimalsHumansMolecular BiologyWnt Signaling PathwayBone mineralMesenchymal Stem CellsGeneral Medicine021001 nanoscience & nanotechnologyAntigens Differentiationdigestive system diseasesMicrospheresCell biologyRatsPLGA030104 developmental biologychemistryGene Expression RegulationStrontiumSclerostinAlkaline phosphatase0210 nano-technologyBiotechnologyBiomedical engineeringActa biomaterialia
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Carbon based nanomaterials for tissue engineering of bone: Building new bone on small black scaffolds: A review.

2019

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0301 basic medicineScaffoldCarbon nanotubesNanotechnologyCarbon nanotubeReview ArticleBone tissuelaw.inventionNanodiamondsScaffold03 medical and health sciences0302 clinical medicineTissue engineeringlawBone cellmedicineCarbon dotsTissue engineeringlcsh:Science (General)BoneCarbon nanomaterialsComputingMethodologies_COMPUTERGRAPHICSGraphene oxidelcsh:R5-920MultidisciplinaryChemistryRegeneration (biology)030104 developmental biologymedicine.anatomical_structure030220 oncology & carcinogenesisCarbon nanomaterialsFullerenesStem celllcsh:Medicine (General)lcsh:Q1-390Journal of advanced research
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Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro

2018

Abstract Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and gene expression in SaOS-2 cells. Material and Methods: SaOS-2 deposition of mineralizing matrix in response to increasing doses of ST (0-1000 nM) was evaluated through Alizarin Red S and Calcein Green staining techniques at 6, 12 and 24 days. Gene expression of runt-related transcription factor 2 (RUNX2), vitamin D receptor (VDR), osteopon…

0301 basic medicineTime FactorsBone matrixCore Binding Factor Alpha 1 SubunitReal-Time Polymerase Chain ReactionCalcitriol receptorBone remodelingCalcificationAndrology03 medical and health sciencesAnabolic AgentsCalcification PhysiologicOsteogenesisCell Line TumorBone cellHumansOsteonectinOsteopontinGeneral DentistryBone mineralAnalysis of VarianceOsteoblastsbiologyChemistryReproducibility of Resultslcsh:RK1-715RUNX2Apposition030104 developmental biologylcsh:DentistryLinear Modelsbiology.proteinAndrogensReceptors CalcitriolOriginal ArticleOsteopontinGene expressionOsteonectinStanozolol
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SNPs in bone-related miRNAs are associated with the osteoporotic phenotype

2017

AbstractBiogenesis and function of microRNAs can be influenced by genetic variants in the pri-miRNA sequences leading to phenotypic variability. This study aims to identify single nucleotide polymorphisms (SNPs) affecting the expression levels of bone-related mature microRNAs and thus, triggering an osteoporotic phenotype. An association analysis of SNPs located in pri-miRNA sequences with bone mineral density (BMD) was performed in the OSTEOMED2 cohort (n = 2183). Functional studies were performed for assessing the role of BMD-associated miRNAs in bone cells. Two SNPs, rs6430498 in the miR-3679 and rs12512664 in the miR-4274, were significantly associated with femoral neck BMD. Further, we…

0301 basic medicineconformation:Diseases::Wounds and Injuries::Fractures Bone::Hip Fractures [Medical Subject Headings]:Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings]Polimorfismo de nucleótido simpleGene ExpressionboneOsteoblastosDensidad ósea:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Cohort StudiesGene Frequencysingle nucleotide polymorphismBone DensityBone cellOssosgeneticsFracturas osteoporóticasCells CulturedGeneticsBone mineralMicroARNsMultidisciplinarymicroRNAbiologyQalleleR:Diseases::Wounds and Injuries::Fractures Bone::Osteoporotic Fractures [Medical Subject Headings]clinical trialMiddle Agedcohort analysisPhenotypeHumanosFenotipmedicine.anatomical_structureCancellous BoneosteoblastMedicine:Diseases::Musculoskeletal Diseases::Bone Diseases [Medical Subject Headings]:Phenomena and Processes::Genetic Phenomena::Genotype [Medical Subject Headings]:Anatomy::Cells::Connective Tissue Cells::Osteoblasts [Medical Subject Headings]AlelosFenotipomusculoskeletal diseasesmedicine.medical_specialtyGenotypeScienceSingle-nucleotide polymorphismBiologychemistryPolymorphism Single NucleotideArticleBone and Bones:Anatomy::Musculoskeletal System::Skeleton::Bone and Bones::Cancellous Bone [Medical Subject Headings]03 medical and health sciencesCalcification PhysiologicInternal medicinemicroRNAmedicineHumanshumanproceduresAllele:Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Alleles [Medical Subject Headings]AllelesFemoral neckGenetic associationAgedcell culture:Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Bone Density [Medical Subject Headings]:Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism Genetic::Polymorphism Single Nucleotide [Medical Subject Headings]OsteoblastsEnfermedades óseasFracturas de caderaComputational BiologyCuello femoral:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Antisense Elements (Genetics)::RNA Antisense::MicroRNAs [Medical Subject Headings]MicroRNAs030104 developmental biologyEndocrinologymulticenter studybone mineralizationNucleic Acid ConformationOsteoporosispathology:Anatomy::Musculoskeletal System::Skeleton::Bone and Bones::Bones of Lower Extremity::Leg Bones::Femur::Femur Neck [Medical Subject Headings]TranscriptomemetabolismGenotipoFractures
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Myeloma-Induced Alterations of Glutamine Metabolism Impair Bone Microenvironment Niche in Multiple Myeloma Patients

2018

Abstract Multiple myeloma (MM) cells are characterized by tight dependence on the bone marrow (BM) microenvironment that exerts a permissive role on cell growth and survival. In turn, MM cells markedly modify their microenvironment leading, in particular, to the development of osteolytic bone lesions. Recently, we demonstrated that metabolic alterations is a major feature of MM cells showing that BM plasma of MM patients is characterized by lower levels of Glutamine (Gln) and higher levels of Glutamate (Glu) and ammonium when compared with patients with smoldering MM (SMM) and Monoclonal Gammopathy of Uncertain Significance (MGUS). In the majority of MM patients MM cells are Gln-addicted si…

0301 basic medicinemedicine.medical_specialtyStromal cellBone diseaseChemistryImmunologyCell BiologyHematologymedicine.disease030226 pharmacology & pharmacyBiochemistryGlutamine03 medical and health sciences030104 developmental biology0302 clinical medicineEndocrinologymedicine.anatomical_structureCell cultureGlutamine synthetaseInternal medicineBone cellmedicineBone marrowMonoclonal gammopathy of undetermined significanceBlood
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Alginate/silica composite hydrogel as a potential morphogenetically active scaffold for three-dimensional tissue engineering

2013

Pursuing our aim to develop a biomimetic synthetic scaffold suitable for tissue engineering, we embedded bone cells, osteoblast-related SaOS-2 cells and osteoclast-like RAW 264.7 cells, into beads, formed of a Na-alginate-based or a silica-containing Na-alginate-based hydrogel matrix. The beads were incubated either separately (only one cell line in a culture dish) or co-incubated (SaOS-2-containing beads and RAW 264.7 beads). The alginate and alginate/silica hydrogel matrices were found not to impair the viability of the encapsulated cells. In these matrices the SaOS-2 cells retain their capacity to synthesize hydroxyapatite crystallites. The mechanical properties, including surface roughn…

0303 health sciencesScaffoldbiologyChemistryGeneral Chemical EngineeringPetri dishCellAcid phosphatase02 engineering and technologyGeneral Chemistry021001 nanoscience & nanotechnologylaw.invention03 medical and health sciencesmedicine.anatomical_structureChemical engineeringTissue engineeringCell culturelawBone cellSelf-healing hydrogelsbiology.proteinmedicine0210 nano-technology030304 developmental biologyRSC Adv.
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Histopathological and immunohistochemical aspects of bone tissue in aseptic necrosis of the femoral head

2021

Femoral head osteonecrosis, also known as avascular necrosis, is a disease with a multifactorial etiology, characterized by a profound change of bone architecture, which leads to the diminishing of bone resistance and femoral head collapse. The main causes that lead to femoral head necrosis are represented by the decrease of local blood perfusion and increase of intraosseous pressure, because of an excessive development of adipose tissue in the areolas of the trabecular bone tissue in the femoral head. The histopathological and immunohistochemical (IHC) study performed by us showed that most of bone trabeculae were damaged by necrotic-involutive processes, their sizes being reduced, both re…

EmbryologyPathologymedicine.medical_specialtymicrocirculationAdipose tissueAvascular necrosisBone tissueOsteocytesPathology and Forensic MedicineFemoral headFemur Head NecrosisBone cellmedicineHumansavascular necrosisEndosteumOriginal PaperAseptic necrosisbusiness.industryosteonecrosismicrofracturesFemur Headfemoral headCell BiologyGeneral Medicinemedicine.diseasemedicine.anatomical_structureAdipose TissueCancellous BoneBone TrabeculaebusinessDevelopmental BiologyRomanian Journal of Morphology and Embryology
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TRAP-Positive Multinucleated Giant Cells Are Foreign Body Giant Cells Rather Than Osteoclasts: Results From a Split-Mouth Study in Humans

2014

This study compared the material-specific tissue response to the synthetic, hydroxyapatite-based bone substitute material NanoBone (NB) with that of the xenogeneic, bovine-based bone substitute material Bio-Oss (BO). The sinus cavities of 14 human patients were augmented with NB and BO in a split-mouth design. Six months after augmentation, bone biopsies were extracted for histological and histomorphometric investigation prior to dental implant insertion. The following were evaluated: the cellular inflammatory pattern, the induction of multinucleated giant cells, vascularization, the relative amounts of newly formed bone, connective tissue, and the remaining bone substitute material. NB gra…

Giant Cells Foreign-BodyMouthPathologymedicine.medical_specialtyForeign-body giant cellBone substituteChemistryOsteoclastsAnatomyGiant CellsGiant cellBone SubstitutesBone cellmedicineAnimalsHumansCattleOral SurgeryJournal of Oral Implantology
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Characterization of C4-2 Prostate Cancer Bone Metastases and Their Response to Castration

2003

New well-characterized preclinical models of prostate cancer (CaP) bone metastases are needed to improve our understanding of the development of CaP-related bone disease in patients. Here we describe characterization of a model consisting of direct injection of C4–2 cells into tibias. Introduction: Prostate cancer (CaP) has a high proclivity to metastasize to bone. Development and characterization of preclinical models of CaP bone metastases are of high interest. The objective of this study was to characterize C4–2 bone metastases and their response to castration. Materials and Methods: Cell suspensions of C4-2, a subline of LNCaP, were injected directly into the tibias of intact male mice.…

Malemusculoskeletal diseasesmedicine.medical_specialtyTime FactorsBone densityBone diseaseEndocrinology Diabetes and MetabolismLong boneMice SCIDBone and BonesMicechemistry.chemical_compoundProstate cancerBone DensityCell Line TumorInternal medicineBone cellmedicineAnimalsHumansOrthopedics and Sports MedicineCastrationNeoplasm MetastasisBone mineralTibiabusiness.industryProstatic NeoplasmsBone metastasisProstate-Specific Antigenmusculoskeletal systemmedicine.diseaseCastrationmedicine.anatomical_structureEndocrinologychemistryReceptors AndrogenbusinessNeoplasm TransplantationJournal of Bone and Mineral Research
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Inorganic polymeric phosphate/polyphosphate as an inducer of alkaline phosphatase and a modulator of intracellular Ca2+ level in osteoblasts (SaOS-2 …

2011

Inorganic polymeric phosphate is a physiological polymer that accumulates in bone cells. In the present study osteoblast-like SaOS-2 cells were exposed to this polymer, complexed in a 2:1 stoichiometric ratio with Ca(2+), polyP (Ca(2+) salt). At a concentration of 100 μM, polyP (Ca(2+) salt) caused a strong increase in the activity of the alkaline phosphatase and also an induction of the steady-state expression of the gene encoding this enzyme. Comparative experiments showed that polyP (Ca(2+) salt) can efficiently replace β-glycerophosphate in the in vitro hydroxyapatite (HA) biomineralization assay. In the presence of polyP (Ca(2+) salt) the cells extensively form HA crystallites, which r…

Materials scienceBiomedical EngineeringSalt (chemistry)BiochemistryCell LinePhosphatesBiomaterials03 medical and health scienceschemistry.chemical_compoundBone cellExtracellularHumansMolecular BiologySaos-2 cells030304 developmental biologychemistry.chemical_classification0303 health sciencesOsteoblastsReverse Transcriptase Polymerase Chain ReactionPolyphosphate030302 biochemistry & molecular biologyGeneral MedicinePhosphateAlkaline PhosphataseImmunohistochemistrychemistryBiochemistryEnzyme InductionBiophysicsMicroscopy Electron ScanningAlkaline phosphataseCalciumIntracellularBiotechnologySignal TransductionActa Biomater.
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